Middlebury

 

Presentation Title: the effects of various hormone manipulations on granulosa cell glutathione

Section: Through the Female Eye: Power, Perspective and Procreation III

Location: McCardell Bicentennial Hall, 104

Date & Time: Friday, April 19, 2013 - 2:30pm - 2:45pm

Abstract:

The ovarian follicle provides a unique microenvironment for the development of a healthy oocyte. Antioxidants play a key role in promoting oocyte quality, especially in the face of oxidative stress. My project aims to understand how levels of the antioxidant glutathione respond to various combinations and concentrations of reproductive hormones within bovine granulosa cells that line the wall of the ovarian follicle. These cells are cultured for 6 days in the presence of follicle-stimulating hormone (FSH) and estradiol (E2), followed by assays to quantify levels of glutathione. FSH and E2 appear to have a synergistic effect, an effect that has been observed in rats but is novel in the bovine system. FSH treatment correlates with an increase in glutathione, and the introduction of E2 may provide an additive effect in promoting glutathione levels. Understanding the antioxidant profile of granulosa cells can ultimately be used to improve assisted reproductive technologies.


Type of Presentation: Individual oral

Oral presenters
Presentation Area: Molecular Biology

Presentation Topic: Science & Technology

Arts presenters
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Class Project
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Presenter Information
Presenter(s): McLean, Molly Elizabeth
Major(s): Molecular Biology and Biochemistry
Class Year(s): 2013

Sponsor(s): Combelles, Catherine M.
Dept(s): Molecular Biology and Biochemistry

Moderator: ,