Presentation Title: the effects of various hormone manipulations on granulosa cell glutathione
Section: Through the Female Eye: Power, Perspective and Procreation III
Location: McCardell Bicentennial Hall, 104
Date & Time: Friday, April 19, 2013 - 2:30pm - 2:45pm
The ovarian follicle provides a unique microenvironment for the development of a healthy oocyte. Antioxidants play a key role in promoting oocyte quality, especially in the face of oxidative stress. My project aims to understand how levels of the antioxidant glutathione respond to various combinations and concentrations of reproductive hormones within bovine granulosa cells that line the wall of the ovarian follicle. These cells are cultured for 6 days in the presence of follicle-stimulating hormone (FSH) and estradiol (E2), followed by assays to quantify levels of glutathione. FSH and E2 appear to have a synergistic effect, an effect that has been observed in rats but is novel in the bovine system. FSH treatment correlates with an increase in glutathione, and the introduction of E2 may provide an additive effect in promoting glutathione levels. Understanding the antioxidant profile of granulosa cells can ultimately be used to improve assisted reproductive technologies.
Type of Presentation: Individual oral
Presentation Area: Molecular Biology
Number of presenters:
Presenter(s): McLean, Molly Elizabeth
Major(s): Molecular Biology and Biochemistry
Class Year(s): 2013
Sponsor(s): Combelles, Catherine M.
Dept(s): Molecular Biology and Biochemistry