COVID-19: Essential Information

2020-2021 Martin H. Freeman Lecture

Professor Pablo Sobrado, Ph.D.

Professor, Associate Department Head, Graduate Committee Chair
Virginia Tech

Wednesday, February 10, 2021

Events  11:45 AM to 3:30 PM
Lecture   1:45 PM - 2:45 PM
(click for Zoom Information)

“Targeting novel flavin-dependent enzymes for the treatment of fungal and bacterial infections"
Iron is an essential nutrient for microbial growth as it is required for a large number of enzymatic reactions. Microbes acquire iron from their environment by heme assimilation, reductive iron transport, and by using iron-binding molecules known as siderophores. In Aspergillus fumigatus and several bacteria including Mycobacterium tuberculosis, siderophore biosynthesis is essential for virulence. Siderophores and their biosynthetic enzymes are absent in humans. Thus, enzymes involved in the biosynthesis of siderophore are ideal drug targets. Similarly, cell wall biosynthesis in bacterial and fungi have been validated as a target for the development of antibiotics. This lecture will focus on the studies of flavin-dependent enzymes involved in the biosynthesis of the cell wall and siderophores in M. tuberculosis and A. fumigatus. Specifically, our work focuses on N-monooxygenases and UDP-galactopyranose mutases. Our approach includes biochemical, structural, and computational studies that have allowed the characterization of the mechanism of oxygen activation, covalent flavin intermediates, and cofactor assisted catalysis. The development of a high-throughput screening assays and identification of inhibitors will also be presented.

Dr. Sobrado’s research focuses on the study of the mechanisms of action, regulation, and structure of enzymes. His research is essential to our understanding of the origins of genetic and degenerative diseases and for providing clues for the identification of drugs against infectious diseases, cancers, or metabolic disorders.  Dr. Sobrado will be a great resource for students interested in research, medicine, and graduate school. 

Hosted by the Chemistry & Biochemistry and Computer Science Departments, and the Center for the Comparative Study of Race and Ethnicity

(Click on the poster to see it full size)

Department of Chemistry & Biochemistry

McCardell Bicentennial Hall
276 Bicentennial Way
Middlebury College
Middlebury, VT 05753