AKAP9 is essential for spermatogenesis and sertoli cell maturation in mice.

TitleAKAP9 is essential for spermatogenesis and sertoli cell maturation in mice.
Publication TypeJournal Article
Year of Publication2013
AuthorsSchimenti, KJ, Feuer, SK, Griffin, LB, Graham, NR, Bovet, CA, Hartford, S, Pendola, J, Lessard, C, Schimenti, JC, Ward, JO
JournalGenetics
Volume194
Issue2
Pagination447-57
Date Published2013 Jun
ISSN1943-2631
KeywordsA Kinase Anchor Proteins, Animals, Anti-Mullerian Hormone, Connexin 43, Cyclin-Dependent Kinase Inhibitor p27, Gap Junctions, Male, Meiosis, Mice, Mice, Mutant Strains, Microtubule-Associated Proteins, Organ Specificity, Protein Transport, Sertoli Cells, Spermatogenesis, Spermatozoa, Spindle Apparatus, Thyroid Hormone Receptors alpha, Tight Junctions, Zonula Occludens-1 Protein
Abstract

<p>Mammalian male fertility relies on complex inter- and intracellular signaling during spermatogenesis. Here we describe three alleles of the widely expressed A-kinase anchoring protein 9 (Akap9) gene, all of which cause gametogenic failure and infertility in the absence of marked somatic phenotypes. Akap9 disruption does not affect spindle nucleation or progression of prophase I of meiosis but does inhibit maturation of Sertoli cells, which continue to express the immaturity markers anti-Mullerian hormone and thyroid hormone receptor alpha in adults and fail to express the maturation marker p27(Kip1). Furthermore, gap and tight junctions essential for blood-testis barrier (BTB) organization are disrupted. Connexin43 (Cx43) and zona occludens-1 are improperly localized in Akap9 mutant testes, and Cx43 fails to compartmentalize germ cells near the BTB. These results identify and support a novel reproductive tissue-specific role for Akap9 in the coordinated regulation of Sertoli cells in the testis.</p>

DOI10.1534/genetics.113.150789
Alternate JournalGenetics
Refereed DesignationRefereed
Full Text
PubMed ID23608191

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